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Image Search Results
Journal: Journal of Cardiovascular Development and Disease
Article Title: Novel lncRNA LNC_000113 Drives the Activation of Pulmonary Adventitial Fibroblasts through Modulating PTEN/Akt/FoxO1 Pathway
doi: 10.3390/jcdd10060262
Figure Lengend Snippet: Increased expression of lncRNA LNC_000113 in PAFs and remodelled pulmonary artery adventitia. ( A ) Expression of lncRNA LNC_000113 in PAFs, PASMCs, and PAECs with Galectin−3 treatment; ( B ) Quantification of the expression of lncRNA LNC_000113 in different stages (1 week and 3 weeks) of MCT rats. ( C ) RNA fluorescence in situ hybridization visualised the localization of the primary expression of lncRNA LNC_000113 in pulmonary artery adventitia (indicated with white arrows). The green asterisk (*) sign indicates the lumen of the pulmonary arteries. Scale bar = 50 μm; ( D ) Quantification of the fluorescence intensity of lncRNA LNC_000113 in healthy controls and MCT rats; Abbreviations: PAFs, pulmonary adventitial fibroblasts; PASMCs, pulmonary artery smooth muscle cells; PAECs, pulmonary arterial endothelial cells. Fluorescent staining: DAPI (blue), lncRNA LNC_000113 (red). Sample size N = 3 for each group in cell experiments of Galectin−3 treatment. Sample size N = 4 for each group in the MCT rat experiments. Significance code: NS stands for not significant; * for p < 0.05 and ** for p < 0.01.
Article Snippet: Primary cultures of PAFs, pulmonary artery smooth muscle cells (PASMCs), and
Techniques: Expressing, Fluorescence, In Situ Hybridization, Staining